RNA

Part:BBa_K199007:Design

Designed by: Olivia Ho-Shing   Group: iGEM09_MoWestern_Davidson   (2009-06-29)

CCAUC Suppressor tRNA (9-bp Anticodon and Produces Serine)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 38
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used the 9-bp anticodon loop that is complementary to the 5-bp codon CCAUC.

Source

De novo synthesis from single-stranded oligos. Based on the paper by Anderson et al. 2002. [http://gcat.davidson.edu/GcatWiki/images/6/6f/AndersonSchultz2002ChemBiol.pdf]

References

[http://gcat.davidson.edu/GcatWiki/images/6/6f/AndersonSchultz2002ChemBiol.pdf (1) Anderson JC, Magliery TJ, Schultz PG. 2002. Exploring the limits of codon and anticodon size. Chemistry & Biology 9:234-44.]